Substrate concentration and yeast catalase

Firstly, more oxygen dissolves in water at low temperatures than at high temperatures, meaning that for the reactions involving low concentrations, more oxygen would have dissolved than in the higher concentrations because of the decreased amount of heat energy given off.

This will slightly affect all the results but as I carried out all the three steps in the same way for all the experiments it should not make any difference to the overall result. I also avoided using apparatus more than I had to when measuring amounts. Convert all readings to seconds to take an average.

Investigation: Enzymes

Doing this could lead to being disqualified from all the subjects that you are taking. Though in theory, this should be the trend, my results did not demonstrate this pattern.

Measuring Enzyme Activity: Yeast Catalase *

So if the apparatus is free of leaks, 22 cm3 of water should be displaced in the measuring cylinder with 10 vol hydrogen peroxide. This is probably due to an experimental error involving one of the factors mentioned above. If there were fewer molecules of hydrogen peroxide, there would have been fewer collisions between molecules of enzyme and substrate, resulting in fewer enzyme-substrate complexes being made.

Oxygen is soluble in water, but dissolves only slowly in water at normal room temperatures. Observation of Catalase Activity 1. Keep in brown bottles because hydrogen peroxide degrades faster in the light. Put the syringe in place in the bung of the flask, but do not push the plunger straight away.

It was very hard to insert the small 5cm3 beaker into the conical flask, and when it came to tipping it over, some of the substrate was still trapped inside the beaker.

I believe that the data also shows strong positive correlation, and there are few outliers, which shows that my results are accurate. A computerized matching algorithm suggests the above articles.

Exploring Enzymes

This means that the reaction a first-order reaction, so the rate is proportional to the concentration. Cut a filter paper disk using a hole punch and soak this in your stock catalase, blot on a paper towel.

As the concentration of hydrogen peroxide increases, the rate of the reaction began to increase. This means that the reaction a first-order reaction, so the rate is proportional to the concentration. Drop the disk into the H2O2.

Effect of Substrate Concentration on the Rate of Activity of Catalase

Convert all readings to seconds to take an average. I decided on the gas syringe method because, as I explained in my section on preliminary work, it measured the volume of gas directly and minimised the volume of oxygen which could potentially dissolve in water.

All the active sites are being used so any extra Hydrogen Peroxide molecules will have to wait until an active site becomes available. There may also be some experimental error which causes the inaccuracies.- The Effect of Substrate Concentration on the Rate of Reaction Between Yeast Catalase and Hydrogen Peroxide Useful info The Enzyme Catalase is a protein molecule which is found in living cells.

Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

It is used to speed up reactions in the cells. Australian science and Mathematics School. Effects of substrate concentration on enzyme activity of catalase from yeast.

Investigation Report. Melanie Bennett. One molecule of catalase can catalyze the decomposition of approximately 4 x 10 7 molecules H 2 O 2 per second!.

In this lab activity, you will be using yeast catalase to observe how increasing and decreasing the concentration of the enzyme and substrate can affect the reaction rate.

In fact, the catalase reaction is dependent on the substrate concentration. If you have an excess of enzyme but not enough substrate, the reaction will be limited by the substrate availability.

Effects of Temperature and Substrate Concentration on Catalase Kristen Clark Biology Lab Section 9/22/14 Brandon Stroup Abstract Catalase is an enzyme found in every living organism and is essential for cells.

Effect of Substrate Concentration on the Rate of Activity of Catalase

The Effect of Substrate Concentration on the Rate of Reaction Between Yeast Catalase and Hydrogen Peroxide Useful info The Enzyme Catalase is a protein molecule which.

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Substrate concentration and yeast catalase
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